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KMID : 0358420070500040612
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Korean Journal of Obstetrics and Gynecology
2007 Volume.50 No. 4 p.612 ~ p.619
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Increase in the Lipid peroxidation and protein oxidation in the venous plasma of women with preterm premature rupture of membranes
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Cho Moon-Kyoung
Kim Yoon-Ha
Kim Cheol-Hong
Kim Ki-Min
Ahn Bong-Whan
Yang Sung-Yeul
Kim Jong-Woon
Song Tae-Bok
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Abstract
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Objective: This study was performed to investigate the lipid peroxide levels and the protein carbonyl groups content in the venous plasma of pregnant women with preterm premature rupture of membranes (PPROM), non-pregnant, and normal pregnant women.
Methods: Samples of venous blood were obtained from women with non pregnancy (n=20), normal pregnancy between 25 and 37 weeks gestation (n=20), and PPROM before 37 completed weeks gestation (n=20). Lipid peroxide levels in the venous plasma of women of each group were measured by thiobarbituric acid reaction. The basal, amoxacillin and moxalactam-induced protein carbonyl contents in the venous plasma of women of each group were determined by the 2,4-dinitrophenylhydrazine (DNPH) method.
Results: 1. Lipid peroxide levels in the venous plasma of PPROM was significantly higher than that of non-pregnant and normal pregnant women (5.66±0.43 vs. 3.78±0.24 vs. 3.56±0.30 nmol/mg protein, p<0.01). 2. Protein carbonyl levels in the venous plasma of PPROM was also significantly higher than that of non-pregnant and normal pregnant women (8.23±0.54 vs. 6.39±0.23 vs. 6.54±0.24 nmol/mg protein, p<0.01). 3. Protein carbonyls formation by moxalactam in the venous plasma of PPROM was significantly higher than that of non-pregnant and normal pregnant women (11.73±0.59 vs. 10.06±0.26 vs. 10.10±0.22 nmol/mg protein, p<0.05). 4. There was no significant difference in protein carbonyls formation by amoxacillin of the venous plasma of pregnant women with PPROM, non-pregnant, and normal pregnant women (5.63±0.41 vs. 5.81±0.43 vs. 5.81±0.39 nmol/mg protein, p>0.05). 5. There were significant positive correlations between lipid peroxide and moxalactam-induced protein carbonyls levels of the venous plasma (p<0.05). There were no significant positive correlations between lipid peroxide and amoxacillin-induced protein carbonyls levels of the venous plasma.
Conclusion: In the venous plasma of pregnant women with PPROM, the lipid peroxidation and the protein carbonyl formation were increased. And moxalactam-induced protein carbonyl levels were increased in PPROM. These results suggest that oxydative stress was increased in pregnant women with PPROM.
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KEYWORD
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Lipid peroxidation, Protein oxidation, Preterm pemature rupture of membranes
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